Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I
PT Duong, HL Collins, M Nickel, S Lund-Katz… - Journal of lipid …, 2006 - ASBMB
The nascent HDL created by ABCA1-mediated efflux of cellular phospholipid (PL) and free
(unesterified) cholesterol (FC) to apolipoprotein AI (apoA-I) has not been defined. To
address this issue, we characterized the lipid particles released when J774 mouse
macrophages and human skin fibroblasts in which ABCA1 is activated are incubated with
human apoA-I. In both cases, three types of nascent HDL containing two, three, or four
molecules of apoA-I per particle are formed. With J774 cells, the predominant species have …
(unesterified) cholesterol (FC) to apolipoprotein AI (apoA-I) has not been defined. To
address this issue, we characterized the lipid particles released when J774 mouse
macrophages and human skin fibroblasts in which ABCA1 is activated are incubated with
human apoA-I. In both cases, three types of nascent HDL containing two, three, or four
molecules of apoA-I per particle are formed. With J774 cells, the predominant species have …
