[HTML][HTML] Lysis reagents, cell numbers, and calculation method influence high-throughput measurement of HDL-mediated cholesterol efflux capacity
JF Schachtl-Riess, S Coassin, C Lamina… - Journal of Lipid …, 2021 - ASBMB
HDL-mediated cholesterol efflux capacity (CEC) may protect against cardiovascular
disease. However, CEC assays are not standardized, hampering their application in large
cohorts and comparison between studies. To improve standardization, we systematically
investigated technical differences between existing protocols that influence assay
performance that have not been previously addressed. CEC was measured in 96-well plates
using J774A. 1 macrophages labeled with BODIPY-cholesterol and incubated for 4 h with …
disease. However, CEC assays are not standardized, hampering their application in large
cohorts and comparison between studies. To improve standardization, we systematically
investigated technical differences between existing protocols that influence assay
performance that have not been previously addressed. CEC was measured in 96-well plates
using J774A. 1 macrophages labeled with BODIPY-cholesterol and incubated for 4 h with …