Direct estimation of HDL-mediated cholesterol efflux capacity from serum
S Kuusisto, MV Holmes, P Ohukainen… - Clinical …, 2019 - academic.oup.com
Clinical chemistry, 2019•academic.oup.com
BACKGROUND HDL-mediated cholesterol efflux capacity (HDL-CEC) is a functional
attribute that may have a protective role in atherogenesis. However, the estimation of HDL-
CEC is based on in vitro cell assays that are laborious and hamper large-scale phenotyping.
METHODS Here, we present a cost-effective high-throughput nuclear magnetic resonance
(NMR) spectroscopy method to estimate HDL-CEC directly from serum. We applied the new
method in a population-based study of 7603 individuals including 574 who developed …
attribute that may have a protective role in atherogenesis. However, the estimation of HDL-
CEC is based on in vitro cell assays that are laborious and hamper large-scale phenotyping.
METHODS Here, we present a cost-effective high-throughput nuclear magnetic resonance
(NMR) spectroscopy method to estimate HDL-CEC directly from serum. We applied the new
method in a population-based study of 7603 individuals including 574 who developed …
BACKGROUND
HDL-mediated cholesterol efflux capacity (HDL-CEC) is a functional attribute that may have a protective role in atherogenesis. However, the estimation of HDL-CEC is based on in vitro cell assays that are laborious and hamper large-scale phenotyping.
METHODS
Here, we present a cost-effective high-throughput nuclear magnetic resonance (NMR) spectroscopy method to estimate HDL-CEC directly from serum. We applied the new method in a population-based study of 7603 individuals including 574 who developed incident coronary heart disease (CHD) during 15 years of follow-up, making this the largest quantitative study for HDL-CEC.
RESULTS
As estimated by NMR-spectroscopy, a 1-SD higher HDL-CEC was associated with a lower risk of incident CHD (hazards ratio, 0.86; 95%CI, 0.79–0.93, adjusted for traditional risk factors and HDL-C). These findings are consistent with published associations based on in vitro cell assays.
CONCLUSIONS
These corroborative large-scale findings provide further support for a potential protective role of HDL-CEC in CHD and substantiate this new method and its future applications.
Oxford University Press