Fetal thymus organ culture
G Anderson, EJ Jenkinson - Cold Spring Harbor Protocols, 2007 - cshprotocols.cshlp.org
G Anderson, EJ Jenkinson
Cold Spring Harbor Protocols, 2007•cshprotocols.cshlp.orgMETHOD The standard fetal thymus organ culture (FTOC) method described in Steps 1 and
2 can be modified to study thymus colonization using hanging drop cultures (Steps 3-5;
Jenkinson et al. 1982). In addition, reaggregate thymus organ cultures (RTOC) can be used,
in which three-dimensional organ cultures are generated from defined mixtures of thymic
stromal cells and thymocytes (Steps 6-16). This latter method is particularly useful in
studying thymic stromal cell function and the development of an individual cohort of …
2 can be modified to study thymus colonization using hanging drop cultures (Steps 3-5;
Jenkinson et al. 1982). In addition, reaggregate thymus organ cultures (RTOC) can be used,
in which three-dimensional organ cultures are generated from defined mixtures of thymic
stromal cells and thymocytes (Steps 6-16). This latter method is particularly useful in
studying thymic stromal cell function and the development of an individual cohort of …
METHOD
The standard fetal thymus organ culture (FTOC) method described in Steps 1 and 2 can be modified to study thymus colonization using hanging drop cultures (Steps 3-5; Jenkinson et al. 1982). In addition, reaggregate thymus organ cultures (RTOC) can be used, in which three-dimensional organ cultures are generated from defined mixtures of thymic stromal cells and thymocytes (Steps 6-16). This latter method is particularly useful in studying thymic stromal cell function and the development of an individual cohort of thymocytes at a defined developmental stage.
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