[CITATION][C] Morphogenetic interactions in the development of the mouse thymus gland
R Auerbach - Developmental biology, 1960 - Elsevier
Developmental biology, 1960•Elsevier
1. Morphogenesis of 12-day mouse embryo thymus glands has been analyzed by in vitro
and transplantation methods. 2. 2. At a glass-clot interface the gland grows and undergoes
characteristic lobulation, but remains predominantly epithelial. In transplants to the anterior
chamber of adult mouse eyes it grows profusely and becomes lymphoidal. 3. 3. The gland
may be separated into epithelial and mesenchymal portions by the use of trypsin. Neither
component can develop in isolation, in vitro or in vivo, but clustering of the two tissues leads …
and transplantation methods. 2. 2. At a glass-clot interface the gland grows and undergoes
characteristic lobulation, but remains predominantly epithelial. In transplants to the anterior
chamber of adult mouse eyes it grows profusely and becomes lymphoidal. 3. 3. The gland
may be separated into epithelial and mesenchymal portions by the use of trypsin. Neither
component can develop in isolation, in vitro or in vivo, but clustering of the two tissues leads …
Abstract
- 1.1. Morphogenesis of 12-day mouse embryo thymus glands has been analyzed by in vitro and transplantation methods.
- 2.2. At a glass-clot interface the gland grows and undergoes characteristic lobulation, but remains predominantly epithelial. In transplants to the anterior chamber of adult mouse eyes it grows profusely and becomes lymphoidal.
- 3.3. The gland may be separated into epithelial and mesenchymal portions by the use of trypsin. Neither component can develop in isolation, in vitro or in vivo, but clustering of the two tissues leads to the restoration of the morphogenetic system, with resultant in vitro lobulation and the in vivo formation of lymphoidal tissue.
- 4.4. Mesenchyme from a variety of embryonic rudiments can exert a morphogenetic effect in vitro, the specific pattern of the epithelial response varying with the source of mesenchyme used. The mesenchymal influence can be exerted across a 20 μ millipore filter barrier.
Elsevier