[HTML][HTML] Mirk/Dyrk1B mediates survival during the differentiation of C2C12Myoblasts
SE Mercer, DZ Ewton, X Deng, S Lim, TR Mazur… - Journal of Biological …, 2005 - ASBMB
The kinase Mirk/dyrk1B is essential for the differentiation of C2C12myoblasts. Mirk
reinforces the G 0/G 1 arrest state inwhich differentiation occurs by directly phosphorylating
and stabilizingp27 Kip1 and destabilizing cyclin D1. We now demonstrate that Mirkis anti-
apoptotic in myoblasts. Knockdown of endogenous Mirk by RNAinterference activated
caspase 3 and decreased myoblast survival by 75%, whereas transient overexpression of
Mirk increased cell survival. Mirk exertsits anti-apoptotic effects during muscle differentiation …
reinforces the G 0/G 1 arrest state inwhich differentiation occurs by directly phosphorylating
and stabilizingp27 Kip1 and destabilizing cyclin D1. We now demonstrate that Mirkis anti-
apoptotic in myoblasts. Knockdown of endogenous Mirk by RNAinterference activated
caspase 3 and decreased myoblast survival by 75%, whereas transient overexpression of
Mirk increased cell survival. Mirk exertsits anti-apoptotic effects during muscle differentiation …