Purification and molecular cloning of a novel essential component of the apolipoprotein B mRNA editing enzyme-complex
H Lellek, R Kirsten, I Diehl, F Apostel, F Buck… - Journal of Biological …, 2000 - ASBMB
Editing of apolipoprotein B (apoB) mRNA requires the catalytic component APOBEC-1
together with" auxiliary" proteins that have not been conclusively characterized so far. Here
we report the purification of these additional components of the apoB mRNA editing enzyme-
complex from rat liver and the cDNA cloning of the novel APOBEC-1-stimulating protein
(ASP). Two proteins copurified into the final active fraction and were characterized by
peptide sequencing and mass spectrometry: KSRP, a 75-kDa protein originally described as …
together with" auxiliary" proteins that have not been conclusively characterized so far. Here
we report the purification of these additional components of the apoB mRNA editing enzyme-
complex from rat liver and the cDNA cloning of the novel APOBEC-1-stimulating protein
(ASP). Two proteins copurified into the final active fraction and were characterized by
peptide sequencing and mass spectrometry: KSRP, a 75-kDa protein originally described as …
