Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor

E Narni-Mancinelli, J Chaix, A Fenis… - Proceedings of the …, 2011 - National Acad Sciences
E Narni-Mancinelli, J Chaix, A Fenis, YM Kerdiles, N Yessaad, A Reynders, C Gregoire…
Proceedings of the National Academy of Sciences, 2011National Acad Sciences
NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of
T cells, and on a population of innate lymphoid cells that produce IL-22 and express the
transcription factor retinoid-related orphan receptor (ROR)-γt, referred to as NK cell receptor
(NKR)+ ROR-γt+ cells. Here we describe Nkp46 iCre knock-in mice in which the gene
encoding the improved Cre (iCre) recombinase was inserted into the Nkp46 locus. This
mouse was used to noninvasively trace cells expressing NKp46 in vivo. Fate mapping …
NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of T cells, and on a population of innate lymphoid cells that produce IL-22 and express the transcription factor retinoid-related orphan receptor (ROR)-γt, referred to as NK cell receptor (NKR)+ROR-γt+ cells. Here we describe Nkp46iCre knock-in mice in which the gene encoding the improved Cre (iCre) recombinase was inserted into the Nkp46 locus. This mouse was used to noninvasively trace cells expressing NKp46 in vivo. Fate mapping experiments demonstrated the stable expression of NKp46 on NK cells and allowed a reappraisal of the sequential steps of NK cell maturation. NKp46 genetic tracing also showed that gut NKR+ROR-γt+ and NK cells represent two distinct lineages. In addition, the genetic heterogeneity of liver NK cells was evidenced. Finally, Nkp46iCre mice also represent a unique mouse model of conditional mutagenesis specifically in NKp46+ cells, paving the way for further developments in the biology of NKp46+ NK, T, and NKR+ROR-γt+ cells.
National Acad Sciences