Isolation and quantitative immunocytochemical characterization of primary myogenic cells and fibroblasts from human skeletal muscle

CC Agley, AM Rowlerson, CP Velloso… - JoVE (Journal of …, 2015 - jove.com
The repair and regeneration of skeletal muscle requires the action of satellite cells, which
are the resident muscle stem cells. These can be isolated from human muscle biopsy
samples using enzymatic digestion and their myogenic properties studied in culture.
Quantitatively, the two main adherent cell types obtained from enzymatic digestion are:(i) the
satellite cells (termed myogenic cells or muscle precursor cells), identified initially as CD56+
and later as CD56+/desmin+ cells and (ii) muscle-derived fibroblasts, identified as CD56 …