Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

C Gardiner, YJ Ferreira, RA Dragovic… - Journal of …, 2013 - Taylor & Francis
C Gardiner, YJ Ferreira, RA Dragovic, CWG Redman, IL Sargent
Journal of extracellular vesicles, 2013Taylor & Francis
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid
sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has
been criticised for a lack of standardisation. We propose the use of silica microspheres for
the calibration of NTA measurements and describe in detail a protocol for the analysis of
EVs by NTA which should minimise many of the sources of variability and imprecision
associated with this technique. Access the supplementary material to this article: Refractive …
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
Access the supplementary material to this article: Refractive index estimation (see Supplementary files under Article Tools online).
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