Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.
K Abremski, R Hoess - Journal of Biological Chemistry, 1984 - ASBMB
Bacteriophage P1 encodes a site-specific recombination system that consists of a site (loxP)
at which recombination occurs and a gene, cre, whose protein product is essential for
recombination. The loxP-Cre recombination event can be studied in greater detail by the use
of an in vitro system that efficiently carries out recombination between two loxP sites. This
paper presents a purification and characterization of the Cre protein (Mr= 35,000), which is
the only protein required for the in vitro reaction. No high energy cofactors are needed. The …
at which recombination occurs and a gene, cre, whose protein product is essential for
recombination. The loxP-Cre recombination event can be studied in greater detail by the use
of an in vitro system that efficiently carries out recombination between two loxP sites. This
paper presents a purification and characterization of the Cre protein (Mr= 35,000), which is
the only protein required for the in vitro reaction. No high energy cofactors are needed. The …