IL-10 inhibits CD28 and ICOS costimulations of T cells via src homology 2 domain—containing protein tyrosine phosphatase 1
BACKGROUND: Specific T-cell activation requires T-cell receptor stimulation and the
generation of costimulatory signals. Major costimulatory signals are delivered to T cells by
the interaction of CD28 and inducible costimulator (ICOS). OBJECTIVE: To investigate the
molecular pathways involved in direct T-cell suppression by IL-10. METHODS: T-cell
proliferation analysis, immunoprecipitations, and Western blots were performed after T-cell
receptor and CD28 and ICOS stimulations in the absence or presence of IL-10. Dominant …
generation of costimulatory signals. Major costimulatory signals are delivered to T cells by
the interaction of CD28 and inducible costimulator (ICOS). OBJECTIVE: To investigate the
molecular pathways involved in direct T-cell suppression by IL-10. METHODS: T-cell
proliferation analysis, immunoprecipitations, and Western blots were performed after T-cell
receptor and CD28 and ICOS stimulations in the absence or presence of IL-10. Dominant …