His239Arg SNP of HRAD9 is associated with lung adenocarcinoma
Y Maniwa, M Yoshimura, VP Bermudez… - … Journal of the …, 2006 - Wiley Online Library
Y Maniwa, M Yoshimura, VP Bermudez, K Okada, N Kanomata, C Ohbayashi, Y Nishimura…
Cancer: Interdisciplinary International Journal of the American …, 2006•Wiley Online LibraryBACKGROUND It was previously reported that a functional human (h) Rad9 protein
accumulated in the nuclei of nonsmall cell lung carcinoma (NSCLC) cells. Those
experiments, however, did not examine whether the hRad9 gene was mutated in those cells.
The sequence of the HRAD9 gene in NSCLC cells was investigated. METHODS The
sequence of the HRAD9 was examined in tumor and peripheral normal lung tissues
obtained from 50 lung adenocarcinoma patients during surgery. The expression of its mRNA …
accumulated in the nuclei of nonsmall cell lung carcinoma (NSCLC) cells. Those
experiments, however, did not examine whether the hRad9 gene was mutated in those cells.
The sequence of the HRAD9 gene in NSCLC cells was investigated. METHODS The
sequence of the HRAD9 was examined in tumor and peripheral normal lung tissues
obtained from 50 lung adenocarcinoma patients during surgery. The expression of its mRNA …
BACKGROUND
It was previously reported that a functional human (h) Rad9 protein accumulated in the nuclei of nonsmall cell lung carcinoma (NSCLC) cells. Those experiments, however, did not examine whether the hRad9 gene was mutated in those cells. The sequence of the HRAD9 gene in NSCLC cells was investigated.
METHODS
The sequence of the HRAD9 was examined in tumor and peripheral normal lung tissues obtained from 50 lung adenocarcinoma patients during surgery. The expression of its mRNA using reverse transcription polymerase chain reaction (RT‐PCR) was also examined.
RESULTS
No sequence alterations were detected in the HRAD9 gene, which was found to be normally transcribed in surgically resected lung carcinoma cells. However, in eight (16.0%) cases a single nucleotide polymorphism (SNP) was observed at the second position of codon 239 (His/Arg heterozygous variant) of the gene. This frequency was significantly higher than that found in the normal population.
CONCLUSIONS
Whereas the capacity to produce a functional hRad9 protein was intact in lung adenocarcinoma cells, a nonsynonymous SNP of HRAD9 was detected that might be associated with the development of lung adenocarcinoma. Cancer 2006. © 2006 American Cancer Society.
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