Endoplasmic reticulum chaperones GRP78 and calreticulin prevent oxidative stress, Ca2+ disturbances, and cell death in renal epithelial cells
H Liu, RC Bowes, B van de Water, C Sillence… - Journal of Biological …, 1997 - ASBMB
Activation of stress response genes can impart cellular tolerance to environmental stress.
Iodoacetamide (IDAM) is an alkylating toxicant that up-regulates expression of hsp70 (Liu,
H., Lightfoot, DL, and Stevens, JL (1996) J. Biol. Chem. 271, 4805–4812) and grp78in LLC-
PK1 renal epithelial cells. Therefore, we used IDAM to determine the role of these genes in
tolerance to toxic chemicals. Prior heat shock did not protect cells from IDAM but
pretreatment withtrans-4, 5-dihydroxy-1, 2-dithiane (DTTox), thapsigargin, or tunicamycin …
Iodoacetamide (IDAM) is an alkylating toxicant that up-regulates expression of hsp70 (Liu,
H., Lightfoot, DL, and Stevens, JL (1996) J. Biol. Chem. 271, 4805–4812) and grp78in LLC-
PK1 renal epithelial cells. Therefore, we used IDAM to determine the role of these genes in
tolerance to toxic chemicals. Prior heat shock did not protect cells from IDAM but
pretreatment withtrans-4, 5-dihydroxy-1, 2-dithiane (DTTox), thapsigargin, or tunicamycin …
