[HTML][HTML] Cyclic AMP induces apolipoprotein E binding activity and promotes cholesterol efflux from a macrophage cell line to apolipoprotein acceptors
JD Smith, M Miyata, M Ginsberg, C Grigaux… - Journal of Biological …, 1996 - ASBMB
RAW 264 mouse macrophage cells were stably transfected with human apolipoprotein E
(apoE) expression vectors. Clonal derivatives were characterized for expression of the
human apoE2, apoE3, and apoE4 isoforms. An apoE4-expressing clonal cell line and a non-
expressing clonal control cell line were loaded overnight with either [3 H] cholesterol or [3 H]
choline. The cells were washed and incubated for 24 h in serum-free medium with or without
the addition of 8-bromo-cyclic AMP (8-Br-cAMP). Only the apoE-secreting cells and only in …
(apoE) expression vectors. Clonal derivatives were characterized for expression of the
human apoE2, apoE3, and apoE4 isoforms. An apoE4-expressing clonal cell line and a non-
expressing clonal control cell line were loaded overnight with either [3 H] cholesterol or [3 H]
choline. The cells were washed and incubated for 24 h in serum-free medium with or without
the addition of 8-bromo-cyclic AMP (8-Br-cAMP). Only the apoE-secreting cells and only in …
