Detection of endothelin-1 mRNA by RT-PCR in isolated rat renal tubules
S Uchida, F Takemoto, E Ogata, K Kurokawa - … and biophysical research …, 1992 - Elsevier
S Uchida, F Takemoto, E Ogata, K Kurokawa
Biochemical and biophysical research communications, 1992•ElsevierA combination of reverse transcription and polymerase chain reaction was utilized to detect
baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5′ and
3′ primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was
found to be expressed only in cortical through medullary collecting ducts in addition to
glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites
along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and …
baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5′ and
3′ primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was
found to be expressed only in cortical through medullary collecting ducts in addition to
glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites
along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and …
Abstract
A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5′ and 3′ primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.
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