[HTML][HTML] RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor κB in osteoclasts
SV Komarova, MF Pilkington, AF Weidema… - Journal of Biological …, 2003 - ASBMB
RANK ligand (RANKL) induces activation of NFκB, enhancing the formation, resorptive
activity, and survival of osteoclasts. Ca 2+ transduces many signaling events, however, it is
not known whether the actions of RANKL involve Ca 2+ signaling. We investigated the
effects of RANKL on rat osteoclasts using microspectrofluorimetry and patch clamp. RANKL
induced transient elevation of cytosolic free Ca 2+ concentration ([Ca 2+] i) to maxima 220
nm above basal, resulting in activation of Ca 2+-dependent K+ current. RANKL elevated [Ca …
activity, and survival of osteoclasts. Ca 2+ transduces many signaling events, however, it is
not known whether the actions of RANKL involve Ca 2+ signaling. We investigated the
effects of RANKL on rat osteoclasts using microspectrofluorimetry and patch clamp. RANKL
induced transient elevation of cytosolic free Ca 2+ concentration ([Ca 2+] i) to maxima 220
nm above basal, resulting in activation of Ca 2+-dependent K+ current. RANKL elevated [Ca …
