Endocytosis in the inner segment of rod photoreceptors: analysis of Xenopus laevis retinas using horseradish peroxidase
JG Hollyfield, ME Rayborn - Experimental eye research, 1987 - Elsevier
To evaluate endocytosis† in rod photoreceptor inner segments, isolated retinas from
Xenopus laevis were maintained in vitro in the presence of the tracer, horseradish
peroxidase (HRP). HRP-positive vesicles, 110±82 nm in diam.(mean±sd), appeared in the
inner segment cytoplasm within 2 min of incubation. Within 8–15 min, 20–30% of the
vesicles in the ellipsoid were HRP-positive, reaching approx. 50% by 30–60 min and
maintaining this level through 4 hr of culture. In retinas incubated 30 min with HRP and then …
Xenopus laevis were maintained in vitro in the presence of the tracer, horseradish
peroxidase (HRP). HRP-positive vesicles, 110±82 nm in diam.(mean±sd), appeared in the
inner segment cytoplasm within 2 min of incubation. Within 8–15 min, 20–30% of the
vesicles in the ellipsoid were HRP-positive, reaching approx. 50% by 30–60 min and
maintaining this level through 4 hr of culture. In retinas incubated 30 min with HRP and then …
