[HTML][HTML] Isolation and characterization of a folate receptor mRNA-binding trans-factor from human placenta: evidence favoring identity with heterogeneous nuclear …
X Xiao, YS Tang, JY Mackins, XL Sun… - Journal of Biological …, 2001 - ASBMB
The interaction of an 18-basecis-element in the 5′-untranslated region of human folate
receptor (FR)-α mRNA with a cytosolic trans-factor protein is critical for the translation of FR
(Sun, X.-L., and Antony, AC (1996) J. Biol. Chem. 271, 25539–25547). This trans-factor was
isolated to apparent homogeneity as a 43-and 38-kDa doublet from human placenta using
poly (U)-Sepharose, followed by preparative sodium dodecyl sulfate-polyacrylamide gel
electrophoresis and electro-elution as major purification steps. Amino acid microsequencing …
receptor (FR)-α mRNA with a cytosolic trans-factor protein is critical for the translation of FR
(Sun, X.-L., and Antony, AC (1996) J. Biol. Chem. 271, 25539–25547). This trans-factor was
isolated to apparent homogeneity as a 43-and 38-kDa doublet from human placenta using
poly (U)-Sepharose, followed by preparative sodium dodecyl sulfate-polyacrylamide gel
electrophoresis and electro-elution as major purification steps. Amino acid microsequencing …