[HTML][HTML] Evidence that a specific interaction between an 18-base cis-element in the 5′-untranslated region of human folate receptor-α mRNA and a 46-kDa cytosolic …
XL Sun, AC Antony - Journal of Biological Chemistry, 1996 - ASBMB
Folate receptors (FR) are inversely regulated by the extracellular folate concentration at the
translational level in cervical carcinoma cells. Accordingly, the potential for interaction of cis-
elements in FR-α mRNA and trans-factors in these cells was determined. Gel-shift assays
identified two signals that were specifically derived from the interaction of cytosolic proteins
with the 5′-untranslated region of FR-α mRNA. RNase T1 mapping revealed that the RNA
sequences interacting with these proteins were located between nucleotides− 133 to− 116 …
translational level in cervical carcinoma cells. Accordingly, the potential for interaction of cis-
elements in FR-α mRNA and trans-factors in these cells was determined. Gel-shift assays
identified two signals that were specifically derived from the interaction of cytosolic proteins
with the 5′-untranslated region of FR-α mRNA. RNase T1 mapping revealed that the RNA
sequences interacting with these proteins were located between nucleotides− 133 to− 116 …