Cytotoxic T cells to an epitope in the islet autoantigen IA-2 are not disease-specific
K Takahashi, MC Honeyman, LC Harrison - Clinical Immunology, 2001 - Elsevier
Cytotoxic CD8 T lymphocytes (CTL) are effectors of pancreatic islet β-cell destruction in type
1 diabetes but, with the exception of a single report, CTL to islet antigen peptides have not
been identified. We used autologous blood monocyte-derived dendritic cells to elicit HLA-A2-
restricted CTL to a peptide, MVWESGCTV (aa 797–805), that is contiguous with a dominant
CD4 T-cell epitope in the islet antigen tyrosine phosphatase IA-2. IA-2 peptide-specific CTL
activity measured as 51Cr release from autologous lymphoblasts was detected in 2/6 islet …
1 diabetes but, with the exception of a single report, CTL to islet antigen peptides have not
been identified. We used autologous blood monocyte-derived dendritic cells to elicit HLA-A2-
restricted CTL to a peptide, MVWESGCTV (aa 797–805), that is contiguous with a dominant
CD4 T-cell epitope in the islet antigen tyrosine phosphatase IA-2. IA-2 peptide-specific CTL
activity measured as 51Cr release from autologous lymphoblasts was detected in 2/6 islet …