Co-regulation of the mitogen-activated protein kinase, extracellular signal-regulated kinase 1, and the 90-kDa ribosomal S6 kinase in PC12 cells. Distinct effects of the …
We recently characterized the association of the 44-kDa mitogen-activated protein kinase,
also known as extracellular-regulated kinase 1 (ERK1), with the 90-kDa ribosomal S6
kinase (pp90rsk), one of its putative substrates in intact PC12 cells. Using antibodies to
ERK1 that precipitate a functional ERK1. pp90rsk phosphoprotein complex, we demonstrate
here the regulation of both kinases by various stimuli. In mouse fibroblasts expressing
human insulin receptors, insulin and vanadate swiftly stimulated ERK1 activity within 5 min …
also known as extracellular-regulated kinase 1 (ERK1), with the 90-kDa ribosomal S6
kinase (pp90rsk), one of its putative substrates in intact PC12 cells. Using antibodies to
ERK1 that precipitate a functional ERK1. pp90rsk phosphoprotein complex, we demonstrate
here the regulation of both kinases by various stimuli. In mouse fibroblasts expressing
human insulin receptors, insulin and vanadate swiftly stimulated ERK1 activity within 5 min …
