[HTML][HTML] Characterization of a novel Na+-dependent, guanosine-specific, nitrobenzylthioinosine-sensitive transporter in acute promyelocytic leukemia cells
SA Flanagan, KA Meckling-Gill - Journal of Biological Chemistry, 1997 - ASBMB
NB4 cells are the only bona fide in vitro model of human acute promyelocytic leukemia. We
have examined cytidine and guanosine transport in this cell line and characterized a novel
guanosine-specific transporter. Cytidine transport occurred predominately by equilibrative
nitrobenzylthioinosine (NBMPR)-sensitive (es) transport. In the presence of Na+, guanosine
at various concentrations accumulated at least 6-fold above equilibrium. The initial rate of
guanosine transport in Na+ buffer decreased by 75% with the addition of 1 μm NBMPR and …
have examined cytidine and guanosine transport in this cell line and characterized a novel
guanosine-specific transporter. Cytidine transport occurred predominately by equilibrative
nitrobenzylthioinosine (NBMPR)-sensitive (es) transport. In the presence of Na+, guanosine
at various concentrations accumulated at least 6-fold above equilibrium. The initial rate of
guanosine transport in Na+ buffer decreased by 75% with the addition of 1 μm NBMPR and …
